-Aniket Majumdar
Melamine, a compound that is added to adulterated milk and dairy products, can lead to kidney-related diseases and cause kidney failure. The techniques currently used to detect it typically require expensive and sophisticated equipment and highly trained personnel, and can take time. In order to make this process simpler and faster, researchers from the Indian Institute of Science (IISc) have designed a low-cost, hand-held device that can detect melamine in water and milk.
Using this device, the researchers tested a range of concentrations of melamine and were able to detect up to 0.1 parts per million (ppm) of melamine in water and milk, which is much lower than the acceptable limit of 1 ppm. Detection took just four minutes.
The team, led by Sai Siva Gorthi, Associate Professor in the Department of Instrumentation and Applied Physics (IAP) at IISc, devised a fluorometer that can detect the formation of copper nanoparticles on specified double-stranded DNA (dsDNA) templates, says Radhika Nambannor Kunnath, a PhD student in Gorthi’s lab and the lead author of the study. These nanoparticles are inexpensive to synthesise and possess a property called fluorescence in which a material emits light of a different wavelength (colour) when a particular wavelength of light falls on it.
The presence of melamine in the sample disrupts the synthesis of these copper nanoparticles and causes a reduction in the intensity of fluorescence, which is detected by the proposed fluorometer. The natural fluorescent glow of these nanoparticles is very weak and their detection requires expensive and sophisticated spectrometers. In a study conducted in 2019, the same team led by Gorthi had shown a way to enhance this glow so that it can be detected by off-the-shelf photodiodes. In the present work, they used electronic components that were easily available at a lower cost, such as a simple UV LED to illuminate the nanoparticles and a PIN photodiode to detect the fluorescence.
The device can be modified to detect other substances too, such as lead and mercury. “This fluorometer can also be easily adapted to detect other analytes of interest (like biomolecules and proteins) using these copper nanoparticles, by changing the template sequence of the dsDNA and other reaction components,” says Kunnath. The team believes that this can be deployed as a screening tool for environmental and food quality testing.
REFERENCE:
Radhika Nambannor Kunnath, Aravind Venukumar, Sai Siva Gorthi, Handheld fluorometer for in-situ melamine detection via interference synthesis of dsDNA-templated copper nanoparticles, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 235 (2020), 118304
https://www.sciencedirect.com/science/article/abs/pii/S1386142520302821?via%3Dihub
(A) Image of the fluorometer (B) Interior of the prototype (Credit: Authors/Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy)
CONTACT:
Sai Siva Gorthi
Associate Professor
Department of Instrumentation and Applied Physics (IAP)
Indian Institute of Science, Bengaluru
saisiva@iisc.ac.in
Nambannor Kunnath
PhD scholar
Department of Instrumentation and Applied Physics (IAP)
Indian Institute of Science, Bengaluru
radhikan@iisc.ac.in
Aravind Venukumar
Project Associate
Department of Instrumentation and Applied Physics (IAP)
Indian Institute of Science, Bengaluru
varavind@iisc.ac.in
NOTE TO JOURNALISTS:
a) If any of the text in this release is reproduced verbatim, please credit the IISc press release.
b) For any queries about IISc press releases, please write to news@iisc.ac.in or pro@iisc.ac.in.
